Composite

Part:BBa_K4825056:Design

Designed by: Ni Weizhao   Group: iGEM23_GreatBay-SCIE   (2023-10-12)


pTDH3-tHMG1-tTDH1-pPGK1-IDi-tPGK1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3643
    Illegal XhoI site found at 2287
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2143
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2541
    Illegal BsaI.rc site found at 3653


Design Notes

"HMG1, 3-hydroxy-3-methylglutaryl-CoA reductase, is the endogenous gene in yeast. It can catalyze the conversion from hydroxy-3-methylglutaryl-CoA (HMG-CoA) to mevalonate (MVA). By accumulating MVA through the over expression of tHMG1, MVA pathway is expressed more efficiently, and the yield of isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP) would increase, which are both precursors of pinene, hence the yield of pinene can be increased. "


Source

Saccharomyces cerevisiae

References